新宝测速6

• A new technology for the rapid detection of mastitis-causing pathogens in dairy cow was developed. 
• This technology also has the potential to determine the inflammatory state of mastitis.
• This new method can contribute to evidence-based antimicrobial use in actual veterinary settings.

Mastitis is an intra-mammary infection considered to be the most common disease occurring in dairy cows. Microbiological examination is necessary for diagnosis, but until now, such culture-based testing requires more than a day to obtain the results. To develop evidence-based treatment strategies for quick initial diagnosis, we devised a new technique for the rapid determination of the causal pathogens responsible for mastitis.

In clinical settings, rapid determination of infection-causing pathogens is performed by direct Gram staining of the sputum, urine and other specimens. In this study, we investigated the sensitivity, specificity, and detection limit of Gram staining of milk precipitates (milk Gram stain, MGS, see Fig. 1) to detect bovine mastitis-causing pathogens in milk, as well as the potential of MGS to diagnose inflammation by counting polymorphonuclear leukocytes (PMN). The results showed that it was comparable to the usefulness of Gram staining used in routine clinical settings and was found to be useful in the diagnosis of mastitis in veterinary settings. In addition, it was also found that the higher the PMN percentage in the Gram-stained microscopic images, the lower was the milk concentration of interleukin 10, an anti-inflammatory cytokine.

• Journal: Frontiers in Veterinary Science
• Title: Determining causal pathogens and inflammatory state of mastitis in dairy cows via Gram staining of precipitates in milk
• Authors: Naoki Suzuki and Naoki Isobe
• DOI: 10.3389/fvets.2024.1492564